Target-Site Mutations and Glutathione S-Transferases Are Associated with Acequinocyl and Pyridaben Resistance in the Two-Spotted Spider Mite Tetranychus urticae (Acari: Tetranychidae).
Identifieur interne : 000037 ( Main/Exploration ); précédent : 000036; suivant : 000038Target-Site Mutations and Glutathione S-Transferases Are Associated with Acequinocyl and Pyridaben Resistance in the Two-Spotted Spider Mite Tetranychus urticae (Acari: Tetranychidae).
Auteurs : Jihye Choi [Corée du Sud] ; Hyun-Na Koo [Corée du Sud] ; Sung Il Kim [Corée du Sud] ; Bueyong Park [Corée du Sud] ; Hyunkyung Kim [Corée du Sud] ; Gil-Hah Kim [Corée du Sud]Source :
- Insects [ 2075-4450 ] ; 2020.
Abstract
The two-spotted spider mite Tetranychus urticae is a difficult-to-control pest due to its short life cycle and rapid resistance development. In this study, we characterized field strains collected in 2001 and 2003 that were selected for acequinocyl resistance (AR) and pyridaben resistance (PR), respectively. These strains displayed resistance ratios of 1798.6 (susceptible vs. AR) and 5555.6 (susceptible vs. PR), respectively, and were screened for cross-resistance against several currently used acaricides. The AR strain exhibited pyridaben cross-resistance, but the PR strain showed no cross-resistance. The AR strain exhibited point mutations in cytb (I256V, N321S) and PSST (H92R). In contrast, the PR strain exhibited the H92R but not the I256V and N321S point mutations. In some cases increased glutathione S-transferase (GST) activity has previously been linked to enhanced detoxification. The AR strain exhibited approximately 2.3-, 1.8-, and 2.2-fold increased GST activity against 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), and 4-nitrobenzyl chloride (NBC), respectively. Among the five GST subclass genes (delta, omega, mu, zeta, and kappa), the relative expression of delta class GSTs in the AR strain were significantly higher than the PR and susceptible strain. These results suggest that the I256V and N321S mutations and the increased GST metabolism and GST delta overexpression might be related to acequinocyl resistance in T. urticae.
DOI: 10.3390/insects11080511
PubMed: 32784738
PubMed Central: PMC7469217
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Target-Site Mutations and Glutathione <i>S</i>-Transferases Are Associated with Acequinocyl and Pyridaben Resistance in the Two-Spotted Spider Mite <i>Tetranychus urticae</i> (Acari: Tetranychidae).</title><author><name sortKey="Choi, Jihye" sort="Choi, Jihye" uniqKey="Choi J" first="Jihye" last="Choi">Jihye Choi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Koo, Hyun Na" sort="Koo, Hyun Na" uniqKey="Koo H" first="Hyun-Na" last="Koo">Hyun-Na Koo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Sung Il" sort="Kim, Sung Il" uniqKey="Kim S" first="Sung Il" last="Kim">Sung Il Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Park, Bueyong" sort="Park, Bueyong" uniqKey="Park B" first="Bueyong" last="Park">Bueyong Park</name><affiliation wicri:level="1"><nlm:affiliation>Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Crop Protection Division, National Institute of Agricultural Science, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hyunkyung" sort="Kim, Hyunkyung" uniqKey="Kim H" first="Hyunkyung" last="Kim">Hyunkyung Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Gil Hah" sort="Kim, Gil Hah" uniqKey="Kim G" first="Gil-Hah" last="Kim">Gil-Hah Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32784738</idno><idno type="pmid">32784738</idno><idno type="doi">10.3390/insects11080511</idno><idno type="pmc">PMC7469217</idno><idno type="wicri:Area/Main/Corpus">000062</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000062</idno><idno type="wicri:Area/Main/Curation">000062</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000062</idno><idno type="wicri:Area/Main/Exploration">000062</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Target-Site Mutations and Glutathione <i>S</i>-Transferases Are Associated with Acequinocyl and Pyridaben Resistance in the Two-Spotted Spider Mite <i>Tetranychus urticae</i> (Acari: Tetranychidae).</title><author><name sortKey="Choi, Jihye" sort="Choi, Jihye" uniqKey="Choi J" first="Jihye" last="Choi">Jihye Choi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Koo, Hyun Na" sort="Koo, Hyun Na" uniqKey="Koo H" first="Hyun-Na" last="Koo">Hyun-Na Koo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Sung Il" sort="Kim, Sung Il" uniqKey="Kim S" first="Sung Il" last="Kim">Sung Il Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Park, Bueyong" sort="Park, Bueyong" uniqKey="Park B" first="Bueyong" last="Park">Bueyong Park</name><affiliation wicri:level="1"><nlm:affiliation>Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Crop Protection Division, National Institute of Agricultural Science, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hyunkyung" sort="Kim, Hyunkyung" uniqKey="Kim H" first="Hyunkyung" last="Kim">Hyunkyung Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Gil Hah" sort="Kim, Gil Hah" uniqKey="Kim G" first="Gil-Hah" last="Kim">Gil-Hah Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644</wicri:regionArea><wicri:noRegion>Cheongju 28644</wicri:noRegion></affiliation></author></analytic><series><title level="j">Insects</title><idno type="ISSN">2075-4450</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The two-spotted spider mite <i>Tetranychus urticae</i> is a difficult-to-control pest due to its short life cycle and rapid resistance development. In this study, we characterized field strains collected in 2001 and 2003 that were selected for acequinocyl resistance (AR) and pyridaben resistance (PR), respectively. These strains displayed resistance ratios of 1798.6 (susceptible vs. AR) and 5555.6 (susceptible vs. PR), respectively, and were screened for cross-resistance against several currently used acaricides. The AR strain exhibited pyridaben cross-resistance, but the PR strain showed no cross-resistance. The AR strain exhibited point mutations in <i>cytb</i> (I256V, N321S) and PSST (H92R). In contrast, the PR strain exhibited the H92R but not the I256V and N321S point mutations. In some cases increased glutathione <i>S</i>-transferase (GST) activity has previously been linked to enhanced detoxification. The AR strain exhibited approximately 2.3-, 1.8-, and 2.2-fold increased GST activity against 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), and 4-nitrobenzyl chloride (NBC), respectively. Among the five GST subclass genes (delta, omega, mu, zeta, and kappa), the relative expression of delta class GSTs in the AR strain were significantly higher than the PR and susceptible strain. These results suggest that the I256V and N321S mutations and the increased GST metabolism and GST delta overexpression might be related to acequinocyl resistance in <i>T. urticae</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32784738</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2075-4450</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>8</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>07</Day></PubDate></JournalIssue><Title>Insects</Title><ISOAbbreviation>Insects</ISOAbbreviation></Journal><ArticleTitle>Target-Site Mutations and Glutathione <i>S</i>-Transferases Are Associated with Acequinocyl and Pyridaben Resistance in the Two-Spotted Spider Mite <i>Tetranychus urticae</i> (Acari: Tetranychidae).</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E511</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/insects11080511</ELocationID><Abstract><AbstractText>The two-spotted spider mite <i>Tetranychus urticae</i> is a difficult-to-control pest due to its short life cycle and rapid resistance development. In this study, we characterized field strains collected in 2001 and 2003 that were selected for acequinocyl resistance (AR) and pyridaben resistance (PR), respectively. These strains displayed resistance ratios of 1798.6 (susceptible vs. AR) and 5555.6 (susceptible vs. PR), respectively, and were screened for cross-resistance against several currently used acaricides. The AR strain exhibited pyridaben cross-resistance, but the PR strain showed no cross-resistance. The AR strain exhibited point mutations in <i>cytb</i> (I256V, N321S) and PSST (H92R). In contrast, the PR strain exhibited the H92R but not the I256V and N321S point mutations. In some cases increased glutathione <i>S</i>-transferase (GST) activity has previously been linked to enhanced detoxification. The AR strain exhibited approximately 2.3-, 1.8-, and 2.2-fold increased GST activity against 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), and 4-nitrobenzyl chloride (NBC), respectively. Among the five GST subclass genes (delta, omega, mu, zeta, and kappa), the relative expression of delta class GSTs in the AR strain were significantly higher than the PR and susceptible strain. These results suggest that the I256V and N321S mutations and the increased GST metabolism and GST delta overexpression might be related to acequinocyl resistance in <i>T. urticae</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Jihye</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koo</LastName><ForeName>Hyun-Na</ForeName><Initials>HN</Initials><AffiliationInfo><Affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Sung Il</ForeName><Initials>SI</Initials><AffiliationInfo><Affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Bueyong</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0002-5740-2241</Identifier><AffiliationInfo><Affiliation>Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hyunkyung</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Gil-Hah</ForeName><Initials>GH</Initials><AffiliationInfo><Affiliation>Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Insects</MedlineTA><NlmUniqueID>101574235</NlmUniqueID><ISSNLinking>2075-4450</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Tetranychus urticae</Keyword><Keyword MajorTopicYN="N">acequinocyl</Keyword><Keyword MajorTopicYN="N">glutathione S-transferase</Keyword><Keyword MajorTopicYN="N">point mutation</Keyword><Keyword MajorTopicYN="N">pyridaben</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>08</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32784738</ArticleId><ArticleId IdType="pii">insects11080511</ArticleId><ArticleId IdType="doi">10.3390/insects11080511</ArticleId><ArticleId IdType="pmc">PMC7469217</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Pest Manag Sci. 2010 Feb;66(2):220-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19894225</ArticleId></ArticleIdList></Reference><Reference><Citation>Pestic Biochem Physiol. 2015 Jun;121:122-8</Citation><ArticleIdList><ArticleId IdType="pubmed">26047120</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2016 Jul;1857(7):902-14</Citation><ArticleIdList><ArticleId IdType="pubmed">26921811</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Soc Trans. 1994 Feb;22(1):230-3</Citation><ArticleIdList><ArticleId IdType="pubmed">8206238</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Entomol. 2007;52:231-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16925478</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1999 Apr 15;27(8):1767-80</Citation><ArticleIdList><ArticleId IdType="pubmed">10101183</ArticleId></ArticleIdList></Reference><Reference><Citation>Insect Biochem Mol Biol. 2012 Nov;42(11):873-80</Citation><ArticleIdList><ArticleId IdType="pubmed">22982600</ArticleId></ArticleIdList></Reference><Reference><Citation>J Econ Entomol. 2019 Mar 21;112(2):835-841</Citation><ArticleIdList><ArticleId IdType="pubmed">30615159</ArticleId></ArticleIdList></Reference><Reference><Citation>Insect Biochem Mol Biol. 2012 Jul;42(7):455-65</Citation><ArticleIdList><ArticleId IdType="pubmed">22465149</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2020 Mar;76(3):1154-1163</Citation><ArticleIdList><ArticleId IdType="pubmed">31599486</ArticleId></ArticleIdList></Reference><Reference><Citation>Insect Biochem Mol Biol. 2010 Aug;40(8):563-72</Citation><ArticleIdList><ArticleId IdType="pubmed">20685616</ArticleId></ArticleIdList></Reference><Reference><Citation>J Econ Entomol. 2001 Dec;94(6):1577-83</Citation><ArticleIdList><ArticleId IdType="pubmed">11777067</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4407-12</Citation><ArticleIdList><ArticleId IdType="pubmed">22393009</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Appl Acarol. 2005;37(3-4):165-71</Citation><ArticleIdList><ArticleId IdType="pubmed">16323048</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Entomol. 2016;61:475-98</Citation><ArticleIdList><ArticleId IdType="pubmed">26982444</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2009 Apr;65(4):404-12</Citation><ArticleIdList><ArticleId IdType="pubmed">19165831</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2005 Feb;61(2):103-10</Citation><ArticleIdList><ArticleId IdType="pubmed">15625668</ArticleId></ArticleIdList></Reference><Reference><Citation>Insect Biochem Mol Biol. 2017 Jan;80:79-90</Citation><ArticleIdList><ArticleId IdType="pubmed">27919778</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>Pestic Biochem Physiol. 2015 Jun;121:61-77</Citation><ArticleIdList><ArticleId IdType="pubmed">26047113</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):5980-5</Citation><ArticleIdList><ArticleId IdType="pubmed">18408150</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2011 Aug;67(8):886-90</Citation><ArticleIdList><ArticleId IdType="pubmed">21538802</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Choi, Jihye" sort="Choi, Jihye" uniqKey="Choi J" first="Jihye" last="Choi">Jihye Choi</name></noRegion><name sortKey="Kim, Gil Hah" sort="Kim, Gil Hah" uniqKey="Kim G" first="Gil-Hah" last="Kim">Gil-Hah Kim</name><name sortKey="Kim, Hyunkyung" sort="Kim, Hyunkyung" uniqKey="Kim H" first="Hyunkyung" last="Kim">Hyunkyung Kim</name><name sortKey="Kim, Sung Il" sort="Kim, Sung Il" uniqKey="Kim S" first="Sung Il" last="Kim">Sung Il Kim</name><name sortKey="Koo, Hyun Na" sort="Koo, Hyun Na" uniqKey="Koo H" first="Hyun-Na" last="Koo">Hyun-Na Koo</name><name sortKey="Park, Bueyong" sort="Park, Bueyong" uniqKey="Park B" first="Bueyong" last="Park">Bueyong Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantGlutaTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000037 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000037 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PlantGlutaTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32784738
|texte= Target-Site Mutations and Glutathione S-Transferases Are Associated with Acequinocyl and Pyridaben Resistance in the Two-Spotted Spider Mite Tetranychus urticae (Acari: Tetranychidae).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32784738" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PlantGlutaTransV1
| This area was generated with Dilib version V0.6.38. |  |